Māori experience disproportionately worse outcomes from infectious diseases compared to non-Māori, and antimicrobial resistance (AMR) contributes to these inequities. The aim of the study reported in this article was to gain insight into Māori experts’ perspectives on AMR using a One Health approach, which incorporates understandings of human, animal and environmental health. Qualitative methods were applied and were guided by principles of Kaupapa Māori research. Four themes were identified:(1) the importance of AMR education for Māori, (2) the connection of mātauranga Māori and AMR, (3) colonisation and its negative impacts on hauora Māori and (4) collaboration across spheres of health as a priority for Māori. Overall, the findings show a need for greater recognition of an approach to AMR that is grounded in te ao Māori. This focus should be a priority for government agencies and healthcare providers across Aotearoa New Zealand in order to work towards health equity for Māori in tackling AMR.